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Fluorescent quantitative PCR is a method for detecting and quantifying target DNA sequences. It selectively amplifies target sequences by specific primers and labels the amplified products using fluorescent probes. The fluorescent signal in the PCR reaction system is proportional to the initial number of the target sequence and can be monitored and recorded by a fluorescence quantitative PCR instrument. The technology has the advantages of high sensitivity, high specificity and wide application range, and is widely used in gene expression research, pathogen detection and molecular diagnosis.